A biotinylated antibody is used to detect the cytokine, which is then quantified using a fluorescent streptavidin-phycoerythrin conjugate
A biotinylated antibody is used to detect the cytokine, which is then quantified using a fluorescent streptavidin-phycoerythrin conjugate. is next transferred to the active site cysteine of any one of a number of ubiquitin conjugating enzymes (E2s), of which there…