in binding buffer (2.5% glycerol, 0.05% Nonidet P-40, 50 mM KCl, 5 mM MgCl2, 1 mM ethylenediaminetetraacetic acid (EDTA), 10 mM Tris, pH 7.6 and 50 ng of poly(dI-dC)). FHL1 with FHL1 small interfering RNA improved the manifestation of these…

**< 0.01, ***< 0.001, unpaired Students test. To determine whether DCs are necessary for the generation of circulating Tfr and Tfh cells, we used BM chimeric mice (CD11c-DTR strain) to deplete DCs. suppressive function of this population was not dependent…